ABSTRACT
Objective: To clarify the optimum conditions for the preparation of mixed materials of oxhide gelatin and honey. Methods: Using the dosage of oxhide gelatin and honey as well as the relative density of refined cream as factors, and the paste forming and fingerprints as control indexes, the central composite design and response surface method was used to optimize the paste preparation process. Results: The extraction process was as follows: 6 times of water amount, soaked for 0.5 h, extracted for three times and each time was 1 h, which was 8 times of water for the first time. The main chromatographic conditions of paste fingerprints were as follows: Mobile phase was acetonitrile-0.1% phosphoric acid gradient [acetonitrile: 10% (0-5 min); 10%-35% (5-15 min); 35%-85% (15-30 min)], and detection wavelengths were 238 and 280 nm. Paste fingerprint: 12 common peaks were found, and the peak No. 7 was identified on honokiol. The RSD values of precision, reproducibility, and stability of each peak relative retention time and relative peak area precision were all less than 3%. The best conditions in the preparation process of cream were as follows: oxhide gelatin (8%-12%), honey (22%-28%), the refined cream and relative density (1.20-1.25). The absolute value of the deviation was less than 3% of paste preparation amplification experiments. Conclusion: This method can be used to ensure that the paste had good formability, and the chemical composition is relatively high.
ABSTRACT
OBJECTIVE: To establish an analysis method for identification of gelatine ingredients in Chinese patent medicines. METHODS: Trypsin was used for hydrolysis of gelatins. The characteristic getalins analyses were identified by rapid resolution liquid chromatography (RRLC) coupled to triple quadruple mass spectrometry (QQQ-MS). Trypsin was used for hydrolysis of gelatins. RESULTS: The established present method was specific, precise and reliable. Gelatine ingredients as prescribed wereas detected for several samples. However, instead of gelatin ingredients as prescribed, unprescribed gelatine ingredients were as detected for some samples. CONCLUSION: The methodology validation is performed and the results are satisfied. The method can be used for identification of donkey-hide gelatin, oxhide gelatin, deer-horn glue and tortoise-shell glue in Chinese patent medicines, and provides a scientific reference for the study of quality control method of gelatin ingredients in Chinese Patent Medicines.